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recombinant bacteria中文是什么意思
- 中文翻譯
- 造句
- 重組細(xì)菌
- recombinant n. 【遺】重組器官，復(fù)合器官。
- bacteria n. pl. (sing. Bacterium ) 1. ...
- recombinant deficient mutant of bacteria 細(xì)菌重組缺陷突變型
- bacteria n. pl. (sing. Bacterium ) 1.細(xì)菌。 2.〔美俚〕拳擊迷。 acid fast bacteria 抗酸細(xì)菌。
- alteplase recombinant 重組阿替普酶
- bacterial recombinant 細(xì)菌重組
- biparental recombinant 二親重組體
- dna recombinant dna重組
- library of recombinant 重組基因文庫(kù)
- patch recombinant 片段重組體
- plasmid recombinant 重組體質(zhì)粒
- recombinant chromosome 重組染色體
- recombinant clones 重組體克隆
- recombinant dna 【生物化學(xué)】重組脫氧核糖核酸。
- recombinant enzymes 重組酶
- recombinant genome 重組體基因組
- recombinant immunotoxin 重組免疫毒素
- recombinant joint 重組接點(diǎn)
- recombinant phage 重組噬菌體
- recombinant plasmid 重組質(zhì)粒
- recombinant point 重組點(diǎn)
- recombinant progeny 重組子代
- recombinant protein 重組蛋白質(zhì)
- recombinant rna 重組rna
- recombinant soluble 重組的可溶性
- These indicated that the porcine il - 6 gene was correctly transcribed and translated in the recombinant bacteria . the fusion protein was recovered from polyacrylamid gel and used to immunize the rabbit , and the liter of rabbit anti - porcine il - 6 serum from immunized rabbit was 1 : 128 , 000
從制備性聚丙烯酰胺凝膠中回收融合蛋白，用mtt法測(cè)定重組蛋白刺激小鼠脾淋巴母細(xì)胞增殖反應(yīng)活性，并以重組蛋白免疫家兔制備抗豬il - 6滴度為128 ， 000的高免兔血清。 - The results were found that the specific 670bp dna bases of cgh was detected by rt - pcr in the recombinant bacteria and a new protein band was found in sds - page with molecular mass of about 50 kda which is consisted of a 23 . 6 kda protein deduced from the cgh gene sequence and gst ( 26 kda )
Rt - pcr分析確證重組質(zhì)粒在大腸桿菌中能正確轉(zhuǎn)錄； sds - page分析證明誘導(dǎo)重組質(zhì)粒表達(dá)了融合蛋白，分子量約為50kd ，與預(yù)期的26kd的gst帶和23 . 6kd的草魚(yú)生長(zhǎng)激素基因編碼蛋白質(zhì)構(gòu)成的融合蛋白大小一致。 - The mrna and protein expression were assayed by rt - pcr and sds - page . the results were found that the specific 740bp dna bases of il - 6 was detected by rt - pcr in the recombinant bacteria and a new protein band was found in sds - page with molecular mass of about 49 kda which is consisted of a 23 kda protein deduced from the il - 6 gene sequence and gst ( 26 kda )
以iptg誘導(dǎo)融合蛋白表達(dá)，經(jīng)rt - pcr檢測(cè)發(fā)現(xiàn)740bp的特異性il - 6條帶；通過(guò)sds - page分析，在49kd處出現(xiàn)gst ? pil6融合蛋白條帶，其表達(dá)量占總細(xì)菌蛋白量的30 ，證明豬il - 6基因得到了正確轉(zhuǎn)錄和表達(dá)。 - The expression conditions of e2 gene in p . pastoris were optimized , the results indicated that the peak obtained after 72 hours ; pattern of inhibition / induction could improve expression level ; the best ph value were between 7 . 5 and 8 . 0 and the optimized methanol - induced concentration was 2 % - 3 % the e2 genes of the prevalent strain ( guangxi yulin strain ) and c strain derived from rabbit spleen tissue were amplified and cloned into e . coli the expression vector pproex - htb respectively , the recombinant plasmids pproex - gxyl and pproex - c were obtained and then were transformed into the dh5a e . coli competent bacteria respectively , the recombinant bacteria could express the major antigen region of e2 gene , the expression yields amount to 35 % and 38 % repectively
豬瘟病毒ez基因的原核表達(dá)： pcr擴(kuò)增出當(dāng)前豬瘟流行野毒株，中國(guó)豬瘟兔化弱毒（ c株）兔脾組織毒ez基因的主要抗原區(qū)，將其克隆到原核大腸桿菌表達(dá)載體pproex htb中誘導(dǎo)表達(dá)，經(jīng)sds page檢測(cè)表明，重組質(zhì)粒能表達(dá)ez基因主要區(qū)蛋白， westernblot檢測(cè)表明，誘導(dǎo)表達(dá)蛋白與豬瘟陽(yáng)性血清發(fā)生特異性反應(yīng)，表達(dá)量為35和38 ，可用于基因工程診斷抗原。
- recombinant bacteriaとは意味：組み換え菌
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